• Immunofluorescence analysis of paraffin-embedded human placenta using PLGF Rabbit polyclonal antibody (STJ118696) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat placenta, using PLGF Rabbit polyclonal antibody (STJ118696) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using PLGF Rabbit polyclonal antibody (STJ118696) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PGF antibody (19-221) (STJ118696)

SKU:
STJ118696

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLGF (19-221) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGF
Gene ID: 5228
Uniprot ID: PLGF_HUMAN
Immunogen Region: 19-221
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-221 of human PLGF (NP_002623.2).
Immunogen Sequence: LPAVPPQQWALSAGNGSSEV EVVPFQEVWGRSYCRALERL VDVVSEYPSEVEHMFSPSCV SLLRCTGCCGDENLHCVPVE TANVTMQLLKIRSGDRPSYV ELTFSQHVRCECRPLREKMK PERRRPKGRGKRRREKQRPT DCHLCGDAVPRR
Tissue Specificity While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
Post Translational Modifications N-glycosylated.
Function Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.
Protein Name Placenta Growth Factor
Plgf
Database Links Reactome: R-HSA-194313
Reactome: R-HSA-195399
Cellular Localisation Secreted
The Three Isoforms Are Secreted But Plgf-2 Appears To Remain Cell Attached Unless Released By Heparin
Alternative Antibody Names Anti-Placenta Growth Factor antibody
Anti-Plgf antibody
Anti-PGF antibody
Anti-PGFL antibody
Anti-PLGF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance