• Immunofluorescence analysis of MCF7 cells using PGAM5 Rabbit polyclonal antibody (STJ118462) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using PGAM5 Rabbit polyclonal antibody (STJ118462) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using PGAM5 Rabbit polyclonal antibody (STJ118462) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using PGAM5 Rabbit polyclonal antibody (STJ118462) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using PGAM5 antibody (STJ118462) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PGAM5 antibody (50-146) (STJ118462)

SKU:
STJ118462

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PGAM5 (50-146) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGAM5
Gene ID: 192111
Uniprot ID: PGAM5_HUMAN
Immunogen Region: 50-146
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-146 of human PGAM5 (NP_001164014.1).
Immunogen Sequence: GARPGPGVWDPNWDRREPLS LINVRKRNVESGEEELASKL DHYKAKATRHIFLIRHSQYH VDGSLEKDRTLTPLGREQAE LTGLRLASLGLKFNKIV
Post Translational Modifications Both isoform 1 and isoform 2 are phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity. Proteolytically cleaved by PARL in response to loss of mitochondrial membrane potential.
Function Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.
Protein Name Serine/Threonine-Protein Phosphatase Pgam5 - Mitochondrial
Bcl-Xl-Binding Protein V68
Phosphoglycerate Mutase Family Member 5
Database Links Reactome: R-HSA-8934903 Q96HS1-2
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Mitochondrion Inner Membrane
Isoform 2 Overexpression Results In The Formation Of Disconnected Punctuate Mitochondria Distributed Throughout The Cytoplasm
Isoform 1 Overexpression Results In The Clustering Of Mitochondria Around The Nucleus
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase Pgam5 - Mitochondrial antibody
Anti-Bcl-Xl-Binding Protein V68 antibody
Anti-Phosphoglycerate Mutase Family Member 5 antibody
Anti-PGAM5 antibody

Information sourced from Uniprot.org

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