• Western blot analysis of various lysates, using PFKL rabbit polyclonal antibody (STJ117838 ) at 1:3070 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of MCF7 cells using PFKL rabbit polyclonal antibody (STJ117838) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PFKL rabbit polyclonal antibody (STJ117838) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using PFKL rabbit polyclonal antibody (STJ117838) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Anti-PFKL antibody (700-780) (STJ117838)

SKU:
STJ117838

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PFKL (700-780) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PFKL
Gene ID: 5211
Uniprot ID: PFKAL_HUMAN
Immunogen Region: 700-780
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 700-780 of human PFKL (NP_002617.3).
Immunogen Sequence: FANAPDSACVIGLKKKAVAF SPVTELKKDTDFEHRMPREQ WWLSLRLMLKMLAQYRISMA AYVSGELEHVTRRTLSMDKG F
Post Translational Modifications GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation.
Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway.
Protein Name Atp-Dependent 6-Phosphofructokinase - Liver Type
Atp-Pfk
Pfk-L
6-Phosphofructokinase Type B
Phosphofructo-1-Kinase Isozyme B
Pfk-B
Phosphohexokinase
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-70171
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Atp-Dependent 6-Phosphofructokinase - Liver Type antibody
Anti-Atp-Pfk antibody
Anti-Pfk-L antibody
Anti-6-Phosphofructokinase Type B antibody
Anti-Phosphofructo-1-Kinase Isozyme B antibody
Anti-Pfk-B antibody
Anti-Phosphohexokinase antibody
Anti-PFKL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance