• Immunohistochemistry analysis of paraffin-embedded mouse stomach using PFKFB3 Rabbit polyclonal antibody (STJ29025) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using PFKFB3 Rabbit polyclonal antibody (STJ29025) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using PFKFB3 Rabbit polyclonal antibody (STJ29025) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PFKFB3 antibody (STJ29025) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using PFKFB3 antibody (STJ29025) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PFKFB3 antibody (130-220) (STJ29025)

SKU:
STJ29025

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PFKFB3 (130-220) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PFKFB3
Gene ID: 5209
Uniprot ID: F263_HUMAN
Immunogen Region: 130-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-220 of human PFKFB3 (NP_004557.1).
Immunogen Sequence: TRERRHMILHFAKENDFKAF FIESVCDDPTVVASNIMEVK ISSPDYKDCNSAEAMDDFMK RISCYEASYQPLDPDKCDRD LSLIKVIDVGR
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylation by AMPK stimulates activity.
Function Catalyzes both the synthesis and degradation of fructose 2,6-bisphosphate.
Protein Name 6-Phosphofructo-2-Kinase/Fructose-2 -6-Bisphosphatase 3
6pf-2-K/Fru-2 -6-P2ase 3
Pfk/Fbpase 3
6pf-2-K/Fru-2 -6-P2ase Brain/Placenta-Type Isozyme
Renal Carcinoma Antigen Ny-Ren-56
Ipfk-2 Includes - 6-Phosphofructo-2-Kinase - Fructose-2 -6-Bisphosphatase
Database Links Reactome: R-HSA-9634600
Alternative Antibody Names Anti-6-Phosphofructo-2-Kinase/Fructose-2 -6-Bisphosphatase 3 antibody
Anti-6pf-2-K/Fru-2 -6-P2ase 3 antibody
Anti-Pfk/Fbpase 3 antibody
Anti-6pf-2-K/Fru-2 -6-P2ase Brain/Placenta-Type Isozyme antibody
Anti-Renal Carcinoma Antigen Ny-Ren-56 antibody
Anti-Ipfk-2 Includes - 6-Phosphofructo-2-Kinase - Fructose-2 -6-Bisphosphatase antibody
Anti-PFKFB3 antibody

Information sourced from Uniprot.org

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