• Confocal imaging of HeLa cells using PFDN5 Rabbit monoclonal antibody (STJ11103552, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of paraffin-embedded Mouse colon tissue using PFDN5 Rabbit monoclonal antibody (STJ11103552, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using PFDN5 Rabbit monoclonal antibody (STJ11103552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PFDN5 antibody (50-154) [S2MR] (STJ11103552)

SKU:
STJ11103552

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PFDN5 (50-154) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PFDN5
Gene ID: 5204
Uniprot ID: PFD5_HUMAN
Immunogen Region: 50-154
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-154 of human PFDN5 (Q99471).
Immunogen Sequence: LNVLNKSNEGKELLVPLTSS MYVPGKLHDVEHVLIDVGTG YYVEKTAEDAKDFFKRKIDF LTKQMEKIQPALQEKHAMKQ AVMEMMSQKIQQLTALGAAQ ATAKA
Tissue Specificity Highly expressed in pancreas and skeletal muscle and moderately in other tissues.
Function Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Represses the transcriptional activity of MYC.
Protein Name Prefoldin Subunit 5
Myc Modulator 1
C-Myc-Binding Protein Mm-1
Database Links Reactome: R-HSA-389957
Cellular Localisation Isoform 1: Nucleus
Isoform 2: Cytoplasm
Isoform 3: Nucleus
Alternative Antibody Names Anti-Prefoldin Subunit 5 antibody
Anti-Myc Modulator 1 antibody
Anti-C-Myc-Binding Protein Mm-1 antibody
Anti-PFDN5 antibody
Anti-MM1 antibody
Anti-PFD5 antibody

Information sourced from Uniprot.org

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