• Immunofluorescence analysis of rat bone marrow using CXCL4/PF4 Rabbit polyclonal antibody (STJ119501) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat bone marrow using CXCL4/PF4 Rabbit polyclonal antibody (STJ119501) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CXCL4/PF4 antibody (STJ119501) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-PF4 antibody (32-101) (STJ119501)

SKU:
STJ119501

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PF4 (32-101) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PF4
Gene ID: 5196
Uniprot ID: PLF4_HUMAN
Immunogen Region: 32-101
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 32-101 of human CXCL4/PF4 (NP_002610.1).
Immunogen Sequence: EAEEDGDLQCLCVKTTSQVR PRHITSLEVIKAGPHCPTAQ LIATLKNGRKICLDLQAPLY KKIIKKLLES
Function Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
Protein Name Platelet Factor 4
Pf-4
C-X-C Motif Chemokine 4
Iroplact
Oncostatin-A Cleaved Into - Platelet Factor 4 - Short Form
Endothelial Cell Growth Inhibitor
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-140875
Reactome: R-HSA-202733
Reactome: R-HSA-380108
Reactome: R-HSA-418594
Reactome: R-HSA-8936459
Cellular Localisation Secreted
Alternative Antibody Names Anti-Platelet Factor 4 antibody
Anti-Pf-4 antibody
Anti-C-X-C Motif Chemokine 4 antibody
Anti-Iroplact antibody
Anti-Oncostatin-A Cleaved Into - Platelet Factor 4 - Short Form antibody
Anti-Endothelial Cell Growth Inhibitor antibody
Anti-PF4 antibody
Anti-CXCL4 antibody
Anti-SCYB4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance