• Anti-PERP antibody (94-193) [S3MR] (STJ11103593)
  • Immunofluorescence analysis of mouse skin cells using PERP Rabbit monoclonal antibody (STJ11103593) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human skin cells using PERP Rabbit monoclonal antibody (STJ11103593) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat skin using PERP Rabbit monoclonal antibody (STJ11103593) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse skin using PERP Rabbit monoclonal antibody (STJ11103593) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PERP Rabbit monoclonal antibody (STJ11103593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PERP antibody (94-193) [S3MR] (STJ11103593)

SKU:
STJ11103593

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PERP (94-193) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PERP
Gene ID: 64065
Uniprot ID: PERP_HUMAN
Immunogen Region: 94-193
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 94-193 of human PERP (Q96FX8).
Immunogen Sequence: LSFFALCGPQMLVFLRVIGG LLALAAVFQIISLVIYPVKY TQTFTLHANPAVTYIYNWAY GFGWAATIILIGCAFFFCCL PNYEDDLLGNAKPRYFYTSA
Tissue Specificity Expressed in skin, heart, placental, liver, pancreas, keratinocytes and dermal fibroblasts.
Function Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway.
Protein Name P53 Apoptosis Effector Related To Pmp-22
Keratinocyte-Associated Protein 1
Kcp-1
P53-Induced Protein Pigpc1
Transmembrane Protein Thw
Database Links Reactome: R-HSA-6803205
Reactome: R-HSA-6809371
Cellular Localisation Cell Junction
Desmosome
Cell Membrane
Multi-Pass Membrane Protein
Associated With Desmosomes
Colocalizes With Krt14 In The Cell Membrane
Alternative Antibody Names Anti-P53 Apoptosis Effector Related To Pmp-22 antibody
Anti-Keratinocyte-Associated Protein 1 antibody
Anti-Kcp-1 antibody
Anti-P53-Induced Protein Pigpc1 antibody
Anti-Transmembrane Protein Thw antibody
Anti-PERP antibody
Anti-KCP1 antibody
Anti-KRTCAP1 antibody
Anti-PIGPC1 antibody
Anti-THW antibody

Information sourced from Uniprot.org

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