Anti-PELO antibody (330-380) {Biotin} (STJ502412)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationBiotin
IsotypeIgG
ImmunogenSynthetic peptide taken within amino acid region 330-380 on human PELOTA protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-PELO (330-380) is suitable for use in Dot Blot, ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Applications | DB/ELISA/IHC/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Monkey/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Biotin |
| Concentration | 0.68-0.70 µg/µl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500DB: 1:4, 000ELISA: 1:4, 000IP: 1:150IHC: 1:50-1:100 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | PELO |
| Gene ID | 53918 |
| Uniprot ID | PELO_HUMAN |
| Immunogen | Synthetic peptide taken within amino acid region 330-380 on human PELOTA protein. |
| Immunogen Region | 330-380 |
Additional Info
| Tissue Specificity | Ubiquitously expressed. |
| Function | Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, PELO recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway. As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy. |
| Protein Name | Protein Pelota HomologHpelotaProtein Dom34 Homolog |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Protein Pelota Homolog antibodyAnti-Hpelota antibodyAnti-Protein Dom34 Homolog antibodyAnti-PELO antibodyAnti-CGI-17 antibody |
Information sourced from Uniprot.org