| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PDHA1 |
| Gene ID: | 5160 |
| Uniprot ID: | ODPA_HUMAN |
| Immunogen Region: | 30-390 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of PDHA1 (NP_000275.1). |
| Immunogen Sequence: | FANDATFEIKKCDLHRLEEG PPVTTVLTREDGLKYYRMMQ TVRRMELKADQLYKQKIIRG FCHLCDGQEACCVGLEAGIN PTDHLITAYRAHGFTFTRGL SVREILAELTGRKGGCAKGK GGSMHMYAKNFYGGNGIVGA QVPLGAGIALACKYNGKDEV CLTLYGDGAANQGQIFEAYN MAALWKLPCIFICENNRYGM GTSVERAAASTDYYKRGDFI PGLRVDGMDILCVREATRF |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3. |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Protein Name | Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - MitochondrialPdhe1-A Type I |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-9837999Reactome: R-HSA-9861559 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial antibodyAnti-Pdhe1-A Type I antibodyAnti-PDHA1 antibodyAnti-PHE1A antibody |
Information sourced from Uniprot.org
