• Immunoprecipitation analysis of 200 Mu g extracts of U-87MG cells using 3 Mu g PDHA1 antibody (STJ11104401). Western blot was performed from the immunoprecipitate using PDHA1 antibody (STJ11104401) at a dilution of 1:1000.
  • Immunofluorescence analysis of U-2 OS cells using PDHA1 Rabbit polyclonal antibody (STJ11104401) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PDHA1 Rabbit polyclonal antibody (STJ11104401) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using PDHA1 Rabbit polyclonal antibody (STJ11104401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PDHA1 antibody (30-390) (STJ11104401)

SKU:
STJ11104401

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha-Somatic Form-Mitochondrial (30-390) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDHA1
Gene ID: 5160
Uniprot ID: ODPA_HUMAN
Immunogen Region: 30-390
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of PDHA1 (NP_000275.1).
Immunogen Sequence: FANDATFEIKKCDLHRLEEG PPVTTVLTREDGLKYYRMMQ TVRRMELKADQLYKQKIIRG FCHLCDGQEACCVGLEAGIN PTDHLITAYRAHGFTFTRGL SVREILAELTGRKGGCAKGK GGSMHMYAKNFYGGNGIVGA QVPLGAGIALACKYNGKDEV CLTLYGDGAANQGQIFEAYN MAALWKLPCIFICENNRYGM GTSVERAAASTDYYKRGDFI PGLRVDGMDILCVREATRF
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3.
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Protein Name Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial
Pdhe1-A Type I
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-389661
Reactome: R-HSA-5362517
Reactome: R-HSA-70268
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial antibody
Anti-Pdhe1-A Type I antibody
Anti-PDHA1 antibody
Anti-PHE1A antibody

Information sourced from Uniprot.org

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