Western blot analysis of various lysates using PDE7A Rabbit polyclonal antibody (STJ117273) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-PDE7A antibody (345-456) (STJ117273)

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STJ117273
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDE7A
Gene ID: 5150
Uniprot ID: PDE7A_HUMAN
Immunogen Region: 345-456
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 345-456 of human PDE7A (NP_002594.1).
Immunogen Sequence: ELSKQWSEKVTEEFFHQGDI EKKYHLGVSPLCDRHTESIA NIQIGFMTYLVEPLFTEWAR FSNTRLSQTMLGHVGLNKAS WKGLQREQSSSEDTDAAFEL NSQLLPQENRLS
Tissue Specificity Isoform PDE7A1: Found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. Isoform PDE7A2: Found abundantly in skeletal muscle and at low levels in heart.
Function Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction.
Protein Name High Affinity 3' -5'-Cyclic-Amp Phosphodiesterase 7a
Hcp1
Tm22
Camp-Specific Phosphodiesterase 7a
Database Links Reactome: R-HSA-418555
Cellular Localisation Isoform Pde7a1: Cytoplasm
Cytosol
Pde7a1 (57 Kda) Is Located Mostly To Soluble Cellular Fractions
Isoform Pde7a2: Cytoplasm
Pde7a2 (50 Kda) Is Located To Particulate Cellular Fractions
Alternative Antibody Names Anti-High Affinity 3' -5'-Cyclic-Amp Phosphodiesterase 7a antibody
Anti-Hcp1 antibody
Anti-Tm22 antibody
Anti-Camp-Specific Phosphodiesterase 7a antibody
Anti-PDE7A antibody

Information sourced from Uniprot.org

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