• Western blot analysis of extracts of various cell lines, using PDE6A antibody (STJ110224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using PDE6A antibody (STJ110224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PDE6A antibody (656-860) (STJ110224)

SKU:
STJ110224

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDE6A (656-860) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDE6A
Gene ID: 5145
Uniprot ID: PDE6A_HUMAN
Immunogen Region: 656-860
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 656-860 of human PDE6A (NP_000431.2).
Immunogen Sequence: EHAIHMMDIAIIATDLALYF KKRTMFQKIVDQSKTYESEQ EWTQYMMLEQTRKEIVMAMM MTACDLSAITKPWEVQSQVA LLVAAEFWEQGDLERTVLQQ NPIPMMDRNKADELPKLQVG FIDFVCTFVYKEFSRFHEEI TPMLDGITNNRKEWKALADE YDAKMKVQEEKKQKQQSAKS AAAGNQPGGNPSPGGATTSK SCCIQ
Function Rod-specific cGMP phosphodiesterase that catalyzes the hydrolysis of 3',5'-cyclic GMP. This protein participates in processes of transmission and amplification of the visual signal.
Protein Name Rod Cgmp-Specific 3' -5'-Cyclic Phosphodiesterase Subunit Alpha
Gmp-Pde Alpha
Pde V-B1
Database Links Reactome: R-HSA-2485179
Reactome: R-HSA-2514859
Reactome: R-HSA-4086398
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Cell Projection
Cilium
Photoreceptor Outer Segment
Alternative Antibody Names Anti-Rod Cgmp-Specific 3' -5'-Cyclic Phosphodiesterase Subunit Alpha antibody
Anti-Gmp-Pde Alpha antibody
Anti-Pde V-B1 antibody
Anti-PDE6A antibody
Anti-PDEA antibody

Information sourced from Uniprot.org

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