• Western blot analysis of lysates from 1) HepG2, 2) HuvEC, 3) COS7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • Western blot analysis of lysates from HepG2 cells, using PCNA Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of COS7 cells using PCNA Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PCNA Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using PCNA Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PCNA antibody (STJ94982)

SKU:
STJ94982
Current Stock:
Host: Rabbit
Applications: WB, IHC-P, ELISA
Reactivity: Human,Mouse,Rat,Monkey
Short Description: Rabbit polyclonal primary antibody against PCNA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Specificity: PCNA polyclonal antibody detects endogenous levels of PCNA protein.
Gene ID: 5111
Immunogen: Synthesized peptide derived from human PCNA
Immunogen Region: 30-110 aa, Internal
Database Links Reactome: R-HSA-110312
Cellular Localisation Nucleus
Colocalizes With Crebbp
Ep300 And Pold1 To Sites Of Dna Damage
Forms Nuclear Foci Representing Sites Of Ongoing Dna Replication And Vary In Morphology And Number During S Phase
Together With Apex2
Is Redistributed In Discrete Nuclear Foci In Presence Of Oxidative Dna Damaging Agents
Protein Name Proliferating Cell Nuclear Antigen
Pcna
Cyclin

Information sourced from uniprot.org

6 months standard