• STJ70781 (2µg/ml) staining of A431 (A) , HEK293 (B) and (1ug/ml) HepG2 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • . STJ70781 (2µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70781 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70781 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-PCK2/PEPCK-M antibody (Internal) (STJ70781)

SKU:
STJ70781-100

£312.50
Shipping:
Free Shipping
Current Stock:
Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PCK2/PEPCK-M (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:4000.
WB: Approx 70kDa band observed in lysates of cell lines A431, HEK293 HepG2 and NIH3T3 (calculated MW of 70.7kDa according to Human NP_004554.3 and 70.5kDa according to Mouse NP_083270.2). R
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PCK2
Gene ID: 5106
Uniprot ID: PCKGM_HUMAN
Immunogen Region: Internal
Accession Number: NP_004554.3; NP_001278485.1
Specificity: No cross-reactivity with PCK1.
Immunogen Sequence: KPWKPGDKEPCAH
Function Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Can play an active role in glyceroneogenesis and gluconeogenesis.
Protein Name Phosphoenolpyruvate Carboxykinase Gtp - Mitochondrial
Pepck-M
Phosphoenolpyruvate Carboxykinase 2 - Mitochondrial
Mtpck2
Database Links Reactome: R-HSA-70263
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Phosphoenolpyruvate Carboxykinase Gtp - Mitochondrial antibody
Anti-Pepck-M antibody
Anti-Phosphoenolpyruvate Carboxykinase 2 - Mitochondrial antibody
Anti-Mtpck2 antibody
Anti-PCK2 antibody
Anti-PEPCK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance