• Western blot analysis of extracts of various cell lines, using PBRM1 antibody (STJ111920) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.
  • Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g PBRM1 rabbit polyclonal antibody (STJ111920). Western blot was performed from the immunoprecipitate using PBRM1 rabbit polyclonal antibody (STJ111920) at a dilition of 1:500.

Anti-PBRM1 antibody (1-230) (STJ111920)

SKU:
STJ111920

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PBRM1 (1-230) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:20-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PBRM1
Gene ID: 55193
Uniprot ID: PB1_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human PBRM1 (NP_060783.3).
Immunogen Sequence: MGSKRRRATSPSSSVSGDFD DGHHSVSTPGPSRKRRRLSN LPTVDPIAVCHELYNTIRDY KDEQGRLLCELFIRAPKRRN QPDYYEVVSQPIDLMKIQQK LKMEEYDDVNLLTADFQLLF NNAKSYYKPDSPEYKAACKL WDLYLRTRNEFVQKGEADDE DDDEDGQDNQGTVTEGSSPA YLKEILEQLLEAIVVATNPS GRLISELFQKLPSKVQYPDY YAIIKEPIDL
Tissue Specificity Widely expressed.
Function Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). Acts as a negative regulator of cell proliferation.
Protein Name Protein Polybromo-1
Hpb1
Brg1-Associated Factor 180
Baf180
Polybromo-1d
Database Links Reactome: R-HSA-3214858
Reactome: R-HSA-8939243
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Protein Polybromo-1 antibody
Anti-Hpb1 antibody
Anti-Brg1-Associated Factor 180 antibody
Anti-Baf180 antibody
Anti-Polybromo-1d antibody
Anti-PBRM1 antibody
Anti-BAF180 antibody
Anti-PB1 antibody

Information sourced from Uniprot.org

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