• Immunofluorescence analysis of U-2 OS cells using PAX6 Rabbit polyclonal antibody (STJ29473) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PAX6 Rabbit polyclonal antibody (STJ29473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-PAX6 antibody (1-422) (STJ29473)

SKU:
STJ29473

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PAX6 (1-422) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAX6
Gene ID: 5080
Uniprot ID: PAX6_HUMAN
Immunogen Region: 1-422
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-422 of human PAX6 (NP_000271.1).
Immunogen Sequence: MQNSHSGVNQLGGVFVNGRP LPDSTRQKIVELAHSGARPC DISRILQVSNGCVSKILGRY YETGSIRPRAIGGSKPRVAT PEVVSKIAQYKRECPSIFAW EIRDRLLSEGVCTNDNIPSV SSINRVLRNLASEKQQMGAD GMYDKLRMLNGQTGSWGTRP GWYPGTSVPGQPTQDGCQQQ EGGGENTNSISSNGEDSDEA QMRLQLKRKLQRNRTSFTQE QIEALEKEFERTHYPDVFA
Tissue Specificity Isoform 1: Expressed in lymphoblasts. Isoform 5a: Weakly expressed in lymphoblasts.
Post Translational Modifications Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.
Function Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells. Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains. Acts as a transcriptional repressor of NFATC1-mediated gene expression.
Protein Name Paired Box Protein Pax-6
Aniridia Type Ii Protein
Oculorhombin
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-381771
Reactome: R-HSA-400511
Reactome: R-HSA-5617472
Cellular Localisation Nucleus
Isoform 1: Nucleus
Isoform 5a: Nucleus
Alternative Antibody Names Anti-Paired Box Protein Pax-6 antibody
Anti-Aniridia Type Ii Protein antibody
Anti-Oculorhombin antibody
Anti-PAX6 antibody
Anti-AN2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance