• Western blot analysis of extracts of various cell lines, using PASK antibody (STJ116338) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of L929 cells using PASK Rabbit polyclonal antibody (STJ116338) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded Human placenta using PASK rabbit polyclonal antibody (STJ116338) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using PASK Rabbit polyclonal antibody (STJ116338) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded Rat testis using PASK rabbit polyclonal antibody (STJ116338) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of PASK in paraffin-embedded Rat testis using PASK Rabbit polyclonal antibody (STJ116338) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using PASK antibody (STJ116338) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PASK in paraffin-embedded Human placenta using PASK Rabbit polyclonal antibody (STJ116338) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of L929 cells using PASK antibody (STJ116338) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PASK Rabbit polyclonal antibody (STJ116338) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PASK antibody (1194-1323) (STJ116338)

SKU:
STJ116338

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PASK (1194-1323) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PASK
Gene ID: 23178
Uniprot ID: PASK_HUMAN
Immunogen Region: 1194-1323
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1194-1323 of human PASK (NP_055963.2).
Immunogen Sequence: YTLVFEENPFCELEETVEAA IHPPYLVSKELMSLVSGLLQ PVPERRTTLEKLVTDPWVTQ PVNLADYTWEEVFRVNKPES GVLSAASLEMGNRSLSDVAQ AQELCGGPVPGEAPNGQGCL HPGDPRLLTS
Tissue Specificity Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails (at protein level).
Post Translational Modifications Autophosphorylated on Thr-1161 and Thr-1165. Autophosphorylation is activated by phospholipids.
Function Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells.however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation.
Protein Name Pas Domain-Containing Serine/Threonine-Protein Kinase
Pas-Kinase
Paskin
Hpask
Cellular Localisation Cytoplasm
Nucleus
Localizes In The Nucleus Of Testis Germ Cells And In The Midpiece Of Sperm Tails
Alternative Antibody Names Anti-Pas Domain-Containing Serine/Threonine-Protein Kinase antibody
Anti-Pas-Kinase antibody
Anti-Paskin antibody
Anti-Hpask antibody
Anti-PASK antibody
Anti-KIAA0135 antibody

Information sourced from Uniprot.org

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