| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PARP2 (N-Term) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 0.02% NaN3 |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:500-1:1000IHCICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PARP2 |
| Gene ID: |
10038 |
| Uniprot ID: |
PARP2_HUMAN |
| Immunogen Region: |
N-Term |
| Specificity: |
Reacts with the N-terminal region of the 62 kDa human PARP-2 protein. |
| Immunogen: |
Synthetic peptide corresponding to amino acids from the N-terminal region of human PARP-2 protein |
| Post Translational Modifications | Auto poly-ADP-ribosylated on serine residues, leading to dissociation of the PARP2-HPF1 complex from chromatin. Poly-ADP-ribosylated by PARP1. Acetylation reduces DNA binding and enzymatic activity. Proteolytically cleaved by caspase-8 (CASP8) in response to apoptosis, leading to its inactivation. |
| Function | Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins.HPF1 conferring serine specificity by completing the PARP2 active site. PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks. HPF1 initiates serine ADP-ribosylation but restricts the polymerase activity of PARP2 in order to limit the length of poly-ADP-ribose chains. Specifically mediates formation of branched poly-ADP-ribosylation. Branched poly-ADP-ribose chains are specifically recognized by some factors, such as APLF. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex. |
| Protein Name | Poly Adp-Ribose Polymerase 2Parp-2Hparp-2Adp-Ribosyltransferase Diphtheria Toxin-Like 2Artd2Dna Adp-Ribosyltransferase Parp2Nad(+ Adp-Ribosyltransferase 2Adprt-2PolyAdp-Ribose Synthase 2Padprt-2Protein Poly-Adp-Ribosyltransferase Parp2 |
| Database Links | Reactome: R-HSA-110362Reactome: R-HSA-5685939Reactome: R-HSA-5696394Reactome: R-HSA-5696395Reactome: R-HSA-5696400 |
| Cellular Localisation | NucleusChromosomeRecruited To Dna Damage Sites In A Parp1-Dependent Process: Recognizes And Binds Poly-Adp-Ribose Chains Produced By Parp1 At Dna Damage Sites Via Its N-TerminusLeading To Its Recruitment |
| Alternative Antibody Names | Anti-Poly Adp-Ribose Polymerase 2 antibodyAnti-Parp-2 antibodyAnti-Hparp-2 antibodyAnti-Adp-Ribosyltransferase Diphtheria Toxin-Like 2 antibodyAnti-Artd2 antibodyAnti-Dna Adp-Ribosyltransferase Parp2 antibodyAnti-Nad(+ Adp-Ribosyltransferase 2 antibodyAnti-Adprt-2 antibodyAnti-PolyAdp-Ribose Synthase 2 antibodyAnti-Padprt-2 antibodyAnti-Protein Poly-Adp-Ribosyltransferase Parp2 antibodyAnti-PARP2 antibodyAnti-ADPRT2 antibodyAnti-ADPRTL2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
