Anti-PARP2 antibody (151-200 aa) (STJ94959)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human PARP2 at the amino acid range 151-200
General Information
| Short Description | Rabbit polyclonal anti-Poly polymerase 2 ADP-ribosyltransferase 2 (151-200 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PARP2 |
| Gene ID | 10038 |
| Uniprot ID | PARP2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human PARP2 at the amino acid range 151-200 |
| Immunogen Region | 151-200 aa |
| Specificity | PARP-2 Polyclonal Antibody detects endogenous levels of PARP-2 protein. |
Additional Info
| Post Translational Modifications | Auto poly-ADP-ribosylated on serine residues, leading to dissociation of the PARP2-HPF1 complex from chromatin. Poly-ADP-ribosylated by PARP1. Acetylation reduces DNA binding and enzymatic activity. Proteolytically cleaved by caspase-8 (CASP8) in response to apoptosis, leading to its inactivation. |
| Function | Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins.HPF1 conferring serine specificity by completing the PARP2 active site. PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks. HPF1 initiates serine ADP-ribosylation but restricts the polymerase activity of PARP2 in order to limit the length of poly-ADP-ribose chains. Specifically mediates formation of branched poly-ADP-ribosylation. Branched poly-ADP-ribose chains are specifically recognized by some factors, such as APLF. In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex. |
| Protein Name | Poly Adp-Ribose Polymerase 2Parp-2Hparp-2Adp-Ribosyltransferase Diphtheria Toxin-Like 2Artd2Dna Adp-Ribosyltransferase Parp2Nad(+ Adp-Ribosyltransferase 2Adprt-2PolyAdp-Ribose Synthase 2Padprt-2Protein Poly-Adp-Ribosyltransferase Parp2 |
| Database Links | Reactome: R-HSA-110362Reactome: R-HSA-5685939Reactome: R-HSA-5696394Reactome: R-HSA-5696395Reactome: R-HSA-5696400 |
| Cellular Localisation | NucleusChromosomeRecruited To Dna Damage Sites In A Parp1-Dependent Process: Recognizes And Binds Poly-Adp-Ribose Chains Produced By Parp1 At Dna Damage Sites Via Its N-TerminusLeading To Its Recruitment |
| Alternative Antibody Names | Anti-Poly Adp-Ribose Polymerase 2 antibodyAnti-Parp-2 antibodyAnti-Hparp-2 antibodyAnti-Adp-Ribosyltransferase Diphtheria Toxin-Like 2 antibodyAnti-Artd2 antibodyAnti-Dna Adp-Ribosyltransferase Parp2 antibodyAnti-Nad(+ Adp-Ribosyltransferase 2 antibodyAnti-Adprt-2 antibodyAnti-PolyAdp-Ribose Synthase 2 antibodyAnti-Padprt-2 antibodyAnti-Protein Poly-Adp-Ribosyltransferase Parp2 antibodyAnti-PARP2 antibodyAnti-ADPRT2 antibodyAnti-ADPRTL2 antibody |
Information sourced from Uniprot.org