| Host: |
Goat |
| Applications: |
WB/IF |
| Reactivity: |
V5 |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-P and V proteins of the Paramyxovirus of simian virus SV5 is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:500-1:5000IF 1:50-1:2000Catarino S Ribeiro-Rodrigues TM Sa Ferreira R et al. Cells 2020 Apr. PMID: 32272685Calado J Santos AR Aires I et al. FEBS Lett 2018 Aug. PMID: 30156268 |
| Storage Instruction: |
For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Specificity: |
Reacts specifically with V5-tagged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation. |
| Immunogen: |
Purified recombinant peptide produced in E. coli. |
| Immunogen Sequence: |
MGKPIPNPLLGLDSTNPMGK PIPNPLLGLDSTNPMGKPIP NPLLGLDSTNPMGKPIPNPL LGLDSTNPMGKPIPNPLLGL DSTNPGRLERPHRD |
| Background | Goat polyclonal antibody to V5 epitope. V5 peptide corresponds to amino acid residues GKPIPNPLLGLDST (95-108 aa) of the P and V proteins of the Paramyxovirus of simian virus SV5. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of SH-SY5Y cells using [KO Validated] Substance P Rabbit polyclonal antibody (STJ11103444) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/164387/365936/STJ11103444_1__64770.1713131389.jpg?c=1 "Immunofluorescence analysis of SH-SY5Y cells using [KO Validated] Substance P Rabbit polyclonal antibody (STJ11103444) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")