• STJ70485 (2µg/ml) staining of Jurkat (A) and U251 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70485 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
  • STJ70485 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-PAR6alpha/PARD6A antibody (C-Term) (STJ70485)

SKU:
STJ70485-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PAR6alpha/PARD6A (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:8000.
WB: Approx 40kDa band observed in lysates of cell lines Jurkat and glioblastoma U251 (calculated MW of 37.4kDa according to NP_058644.1). Recommended concentration: 1-3µg/ml. Primary incuba
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PARD6A
Gene ID: 50855
Uniprot ID: PAR6A_HUMAN
Immunogen Region: C-Term
Accession Number: NP_058644.1; NP_001032358.1
Specificity: This antibody is expected to recognise both reported isoforms (NP_058644.1; NP_001032358.1).
Immunogen Sequence: GSRIRGDGSGFSL
Post Translational Modifications Phosphorylated by the TGF-beta receptor.
Function Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization.
Protein Name Partitioning Defective 6 Homolog Alpha
Par-6
Par-6 Alpha
Par-6a
Par6c
Tax Interaction Protein 40
Tip-40
Database Links Reactome: R-HSA-2173791
Reactome: R-HSA-420029
Reactome: R-HSA-4608870
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013420
Reactome: R-HSA-9013424
Cellular Localisation Cytoplasm
Cell Membrane
Cell Projection
Ruffle
Cell Junction
Tight Junction
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriolar Satellite
Colocalizes With Gtp-Bound Cdc42 Or Rac1 At Membrane Ruffles And With Pard3 And Prkci At Epithelial Tight Junctions
Recruited To The Centrosome By A Microtubule And Dynein-Dynactin-Dependent Mechanism
Alternative Antibody Names Anti-Partitioning Defective 6 Homolog Alpha antibody
Anti-Par-6 antibody
Anti-Par-6 Alpha antibody
Anti-Par-6a antibody
Anti-Par6c antibody
Anti-Tax Interaction Protein 40 antibody
Anti-Tip-40 antibody
Anti-PARD6A antibody
Anti-PAR6A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance