Anti-PAK4 antibody (450-550) (STJ113249)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PAK4 (450-550) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PAK4 |
| Gene ID | 10298 |
| Uniprot ID | PAK4_HUMAN |
| Immunogen Region | 450-550 |
| Immunogen Sequence | HDGRVKLSDFGFCAQVSKEV PRRKSLVGTPYWMAPELISR LPYGPEVDIWSLGIMVIEMV DGEPPYFNEPPLKAMKMIRD NLPPRLKNLHKVSPSLKGFL D |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 450-550 of human PAK4 (NP_005875.1). |
Additional Info
| Tissue Specificity | Highest expression in prostate, testis and colon. |
| Post Translational Modifications | Autophosphorylated on serine residues when activated by CDC42/p21 (Ref.33). Phosphorylated on tyrosine residues upon stimulation of FGFR2. Methylated by SETD6. Polyubiquitinated, leading to its proteasomal degradation. |
| Function | Serine/threonine-protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell adhesion turnover, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN. Promotes kinase-independent stabilization of RHOU, thereby contributing to focal adhesion disassembly during cell migration. |
| Protein Name | Serine/Threonine-Protein Kinase Pak 4P21-Activated Kinase 4Pak-4 |
| Database Links | Reactome: R-HSA-428540Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013406Reactome: R-HSA-9013407Reactome: R-HSA-9013408Reactome: R-HSA-9013409Reactome: R-HSA-9013420Reactome: R-HSA-9013423Reactome: R-HSA-9013424 |
| Cellular Localisation | CytoplasmSeems To Shuttle Between Cytoplasmic Compartments Depending On The Activating EffectorFor ExampleCan Be Found On The Cell Periphery After Activation Of Growth-Factor Or Integrin-Mediated Signaling Pathways |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Pak 4 antibodyAnti-P21-Activated Kinase 4 antibodyAnti-Pak-4 antibodyAnti-PAK4 antibodyAnti-KIAA1142 antibody |
Information sourced from Uniprot.org