• Western blot analysis of lysates from Jurkat cells, using Serpin A5 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of Hela cells using PAI-3 Polyclonal Antibody
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of various cells using PAI-3 Polyclonal Antibody

Anti-PAI-3 antibody (STJ94936)

SKU:
STJ94936

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, ELISA
Reactivity: Human
Short Description: Rabbit polyclonal anti-PAI-3 antibody is suitable for use in Western Blot, Immunohistochemistry and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Specificity: PAI-3 polyclonal antibody detects endogenous levels of PAI-3 protein.
Uniprot ID: IPSP_HUMAN
Gene ID: 5104
Gene Symbol: SERPINA5
Immunogen: Synthesized peptide derived from human PAI-3
Immunogen Region: 230-310 aa, Internal
Post Translational Modifications N- and O-glycosylated. N-glycosylation consists of a mixture of sialylated bi- (including sialyl-Lewis X epitopes), tri- and tetra-antennary complex-type chains.affects the maximal heparin- and thrombomodulin-enhanced rates of thrombin inhibition. O-glycosylated with core 1 or possibly core 8 glycans. Further modified with 2 sialic acid residues. Proteolytically cleaved. Inhibition of proteases is accompanied by formation of a stable enzyme-inhibitor complex and by degradation of the serpin to lower molecular weight derivatives. Proteolytically cleaved at the N-terminus.inhibits slightly the heparin- and thrombomodulin-enhanced rates of thrombin inhibition.
Function Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and proinflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary-type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin.indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue- and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities.has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2.regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as a hydrophobic hormone carrier by its binding to retinoic acid.
Protein Name Plasma Serine Protease Inhibitor
Acrosomal Serine Protease Inhibitor
Plasminogen Activator Inhibitor 3
Pai-3
Pai3
Protein C Inhibitor
Pci
Serpin A5
Database Links Reactome: R-HSA-140837
Reactome: R-HSA-140875
Cellular Localisation Secreted
Extracellular Space
Localized On The Plasma Membrane Overlying The Acrosomal Head Of Spermatozoa Of Ependymal Spermatozoa And Ejaculated Sperm
Localized At The Equatorial Segment Of Acrosome-Reacted Spematozoa
Localized In Alpha Granules In Resting Platelets And On The External Plasma Membrane And Within The Surface-Connected Cannalicular System In Activated Platelets
Alternative Antibody Names Anti-Plasma Serine Protease Inhibitor antibody
Anti-Acrosomal Serine Protease Inhibitor antibody
Anti-Plasminogen Activator Inhibitor 3 antibody
Anti-Pai-3 antibody
Anti-Pai3 antibody
Anti-Protein C Inhibitor antibody
Anti-Pci antibody
Anti-Serpin A5 antibody
Anti-SERPINA5 antibody
Anti-PCI antibody
Anti-PLANH3 antibody
Anti-PROCI antibody

Information sourced from Uniprot.org

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