| Host: | Goat |
| Applications: | Pep-ELISA/IF/IHC |
| Reactivity: | Human/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-P4HA1 (Internal) is suitable for use in ELISA, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.IHC: Paraffin embedded Human Spleen. Recommended concentration: 7µg/ml.IF: Strong expression of the protein seen in the ER and cytoplasm of A431 cells. Recommended concentration: 10µ |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | P4HA1 |
| Gene ID: | 5033 |
| Uniprot ID: | P4HA1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000908.2; NP_001017962.1 |
| Specificity: | This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1). |
| Immunogen Sequence: | DFARKDEPDAFKE |
| Function | Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. |
| Protein Name | Prolyl 4-Hydroxylase Subunit Alpha-14-Ph Alpha-1Procollagen-Proline -2-Oxoglutarate-4-Dioxygenase Subunit Alpha-1 |
| Database Links | Reactome: R-HSA-1650814 |
| Cellular Localisation | Endoplasmic Reticulum Lumen |
| Alternative Antibody Names | Anti-Prolyl 4-Hydroxylase Subunit Alpha-1 antibodyAnti-4-Ph Alpha-1 antibodyAnti-Procollagen-Proline -2-Oxoglutarate-4-Dioxygenase Subunit Alpha-1 antibodyAnti-P4HA1 antibodyAnti-P4HA antibody |
Information sourced from Uniprot.org