• Immunofluorescence analysis of L929 cells using P4HA1 Rabbit polyclonal antibody (STJ112556) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Rat skeletal muscle, using P4HA1 antibody (STJ112556) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120.
  • Western blot analysis of extracts of various cell lines, using P4HA1 antibody (STJ112556) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-P4HA1 antibody (235-534) (STJ112556)

SKU:
STJ112556

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-P4HA1 (235-534) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: P4HA1
Gene ID: 5033
Uniprot ID: P4HA1_HUMAN
Immunogen Region: 235-534
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 235-534 of human P4HA1 (NP_001017962.1).
Immunogen Sequence: DPEHQRANGNLKYFEYIMAK EKDVNKSASDDQSDQKTTPK KKGVAVDYLPERQKYEMLCR GEGIKMTPRRQKKLFCRYHD GNRNPKFILAPAKQEDEWDK PRIIRFHDIISDAEIEIVKD LAKPRLRRATISNPITGDLE TVHYRISKSAWLSGYENPVV SRINMRIQDLTGLDVSTAEE LQVANYGVGGQYEPHFDFAR KDEPDAFKELGTGNRIATWL FYMSDVSAGGATVFPEVGA
Tissue Specificity Expressed in the heart, liver, skeletal muscle, kidney, placenta, lung and pancreas.
Function Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Protein Name Prolyl 4-Hydroxylase Subunit Alpha-1
4-Ph Alpha-1
Procollagen-Proline -2-Oxoglutarate-4-Dioxygenase Subunit Alpha-1
Database Links Reactome: R-HSA-1650814
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Prolyl 4-Hydroxylase Subunit Alpha-1 antibody
Anti-4-Ph Alpha-1 antibody
Anti-Procollagen-Proline -2-Oxoglutarate-4-Dioxygenase Subunit Alpha-1 antibody
Anti-P4HA1 antibody
Anti-P4HA antibody

Information sourced from Uniprot.org

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