• Immunofluorescence analysis of MCF7 cells using P2RX4 antibody (STJ28765). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using P2RX4 antibody (STJ28765) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Anti-P2RX4 antibody (STJ28765)

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Host: Rabbit
Applications: WB, IF
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal primary antibody against P2RX4.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IF: 1:10-1:100
Uniprot ID: P2RX4_HUMAN
Gene ID: 5025
Gene Symbol: P2RX4
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 55-338 of human P2RX4 (NP_002551.2).
Background The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants, some protein-coding and some not protein-coding, have been found for this gene.
Function Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Database Links Reactome: R-HSA-139853
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Protein Name P2x Purinoceptor 4
Atp Receptor
Purinergic Receptor
Alternative Names Anti-P2x Purinoceptor 4 antibody
Anti-P2x4 antibody
Anti-Atp Receptor antibody
Anti-Purinergic Receptor antibody
Anti-P2RX4 antibody

Information sourced from uniprot.org

6 months standard