• STJ73599 (0. 3µg/ml) staining of U937 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73599 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73599 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-OCIL antibody (Internal) (STJ73599)

SKU:
STJ73599-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC/IHC
Reactivity: Human/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-OCIL (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-3.75µg/ml
IF-Strong expression of the protein seen in the cytoplasm of A431 cells. 10µg/ml
FC-Flow cytometric analysis of Jurkat cells. 10ug/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CLEC2D
Gene ID: 29121
Uniprot ID: CLC2D_HUMAN
Immunogen Region: Internal
Accession Number: NP_037401.1; NP_001004419.1; NP_001184246.1
Specificity: This antibody is expected to recognize all isoforms (NP_037401.1; NP_001004419.1; NP_001184246.1).
Immunogen Sequence: LLRYKGPSDH
Post Translational Modifications N-glycosylated.
Function Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon-gamma. Binds high molecular weight sulfated glycosaminoglycans.
Protein Name C-Type Lectin Domain Family 2 Member D
Lectin-Like Nk Cell Receptor
Lectin-Like Transcript 1
Llt-1
Osteoclast Inhibitory Lectin
Database Links Reactome: R-HSA-198933
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Isoform 2: Endoplasmic Reticulum
Isoform 4: Endoplasmic Reticulum
Alternative Antibody Names Anti-C-Type Lectin Domain Family 2 Member D antibody
Anti-Lectin-Like Nk Cell Receptor antibody
Anti-Lectin-Like Transcript 1 antibody
Anti-Llt-1 antibody
Anti-Osteoclast Inhibitory Lectin antibody
Anti-CLEC2D antibody
Anti-CLAX antibody
Anti-LLT1 antibody
Anti-OCIL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance