STJ71825 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Anti-NY-ESO-1 antibody (Internal) (STJ71825)
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| Host: |
Goat |
| Applications: |
Pep-ELISA/IF/FC/MA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : |
Goat polyclonal antibody anti-NY-ESO-1 (Internal) is suitable for use in ELISA, Immunofluorescence, Flow Cytometry and MA research applications. |
| Clonality : |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:16000.
IF: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10µg/ml.FC:Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/m |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_001318.1 |
| Immunogen Sequence: |
AELARRSLAQDAP |
