| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Kinetochore protein Nuf2 (199-249 aa) is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NUF2 |
| Gene ID: | 83540 |
| Uniprot ID: | NUF2_HUMAN |
| Immunogen Region: | 199-249 aa |
| Specificity: | This antibody detects endogenous levels of NUF2 at Human/Mouse/Rat |
| Immunogen: | Synthesized peptide derived from the human NUF2 at the amino acid range 199-249 |
| Function | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. |
| Protein Name | Kinetochore Protein Nuf2 Hnuf2 Hnuf2r Hsnuf2 Cell Division Cycle-Associated Protein 1 |
| Database Links | Reactome: R-HSA-141444 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-5663220 Reactome: R-HSA-68877 Reactome: R-HSA-9648025 |
| Cellular Localisation | Nucleus Chromosome Centromere Kinetochore Localizes To Kinetochores From Late Prophase To Anaphase Localizes Specifically To The Outer Plate Of The Kinetochore Ndc80 Is Required For Efficient Kinetochore Localization |
| Alternative Antibody Names | Anti-Kinetochore Protein Nuf2 antibody Anti-Hnuf2 antibody Anti-Hnuf2r antibody Anti-Hsnuf2 antibody Anti-Cell Division Cycle-Associated Protein 1 antibody Anti-NUF2 antibody Anti-CDCA1 antibody Anti-NUF2R antibody |
Information sourced from Uniprot.org