• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g MTH1 antibody (STJ115293). Western blot was performed from the immunoprecipitate using MTH1 antibody (STJ115293) at a dilution of 1:1000.
  • Western blot analysis of lysates from wild type (WT) and MTH1 knockout (KO) 293T cells, using [KO Validated] MTH1 Rabbit polyclonal antibody (STJ115293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using [KO Validated] MTH1 Rabbit polyclonal antibody (STJ115293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NUDT1 antibody (1-179) (STJ115293)

SKU:
STJ115293

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUDT1 (1-179) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUDT1
Gene ID: 4521
Uniprot ID: 8ODP_HUMAN
Immunogen Region: 1-179
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human MTH1 (NP_945192.1).
Immunogen Sequence: MSGISPQQMGEPEGSWSGKN PGTMGASRLYTLVLVLQPQR VLLGMKKRGFGAGRWNGFGG KVQEGETIEDGARRELQEES GLTVDALHKVGQIVFEFVGE PELMDVHVFCTDSIQGTPVE SDEMRPCWFQLDQIPFKDMW PDDSYWFPLLLQKKKFHGYF KFQGQDTILDYTLREVDTV
Tissue Specificity Widely expressed with highest expression in thymus, testis, embryo and proliferating blood lymphocytes.
Post Translational Modifications The N-terminus is blocked.
Function Oxidized purine nucleoside triphosphate hydrolase which is a prominent sanitizer of the oxidized nucleotide pool. Catalyzes the hydrolysis of 2-oxo-dATP (2-hydroxy-dATP) into 2-oxo-dAMP. Has also a significant hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP and 8-oxo-dATP. Through the hydrolysis of oxidized purine nucleoside triphosphates, prevents their incorporation into DNA and the subsequent transversions A:T to C:G and G:C to T:A. Also catalyzes the hydrolysis of methylated purine nucleoside triphosphate preventing their integration into DNA. Through this antimutagenic activity protects cells from oxidative stress.
Protein Name Oxidized Purine Nucleoside Triphosphate Hydrolase
2-Hydroxy-Datp Diphosphatase
7 -8-Dihydro-8-Oxoguanine Triphosphatase
8-Oxo-Dgtpase
Methylated Purine Nucleoside Triphosphate Hydrolase
Nucleoside Diphosphate-Linked Moiety X Motif 1
Nudix Motif 1
Database Links Reactome: R-HSA-2393930
Cellular Localisation Isoform P18: Cytoplasm
Cytosol
Mitochondrion Matrix
Nucleus
Mostly Present In Cytosol
A Minor Proportion Is Mitochondrial
A Very Small Amount Of The Protein Is Associated With Nuclei
Isoform P26: Mitochondrion Matrix
Alternative Antibody Names Anti-Oxidized Purine Nucleoside Triphosphate Hydrolase antibody
Anti-2-Hydroxy-Datp Diphosphatase antibody
Anti-7 -8-Dihydro-8-Oxoguanine Triphosphatase antibody
Anti-8-Oxo-Dgtpase antibody
Anti-Methylated Purine Nucleoside Triphosphate Hydrolase antibody
Anti-Nucleoside Diphosphate-Linked Moiety X Motif 1 antibody
Anti-Nudix Motif 1 antibody
Anti-NUDT1 antibody
Anti-MTH1 antibody

Information sourced from Uniprot.org

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