Anti-NTMT1 antibody (1-223) (STJ29178)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NTMT1 (1-223) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NTMT1 |
| Gene ID | 28989 |
| Uniprot ID | NTM1A_HUMAN |
| Immunogen Region | 1-223 |
| Immunogen Sequence | MTSEVIEDEKQFYSKAKTYW KQIPPTVDGMLGGYGHISSI DINSSRKFLQRFLREGPNKT GTSCALDCGAGIGRITKRLL LPLFREVDMVDITEDFLVQA KTYLGEEGKRVRNYFCCGLQ DFTPEPDSYDVIWIQWVIGH LTDQHLAEFLRRCKGSLRPN GIIVIKDNMAQEGVILDDVD SSVCRDLDVVRRIICSAGLS LLAEERQENLPDEIYHVYSF ALR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human NTMT1 (NP_054783.2). |
Additional Info
| Function | Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif Ala/Gly/Pro/Ser-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the Ala/Gly/Ser-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by NTMT2-mediated monomethylation. Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1. |
| Protein Name | N-Terminal Xaa-Pro-Lys N-Methyltransferase 1Alpha N-Terminal Protein Methyltransferase 1aMethyltransferase-Like Protein 11aN-Terminal Rcc1 MethyltransferaseX-Pro-Lys N-Terminal Protein Methyltransferase 1aNtm1a Cleaved Into - N-Terminal Xaa-Pro-Lys N-Methyltransferase 1 - N-Terminally Processed |
| Database Links | |
| Cellular Localisation | NucleusPredominantly Nuclear |
| Alternative Antibody Names | Anti-N-Terminal Xaa-Pro-Lys N-Methyltransferase 1 antibodyAnti-Alpha N-Terminal Protein Methyltransferase 1a antibodyAnti-Methyltransferase-Like Protein 11a antibodyAnti-N-Terminal Rcc1 Methyltransferase antibodyAnti-X-Pro-Lys N-Terminal Protein Methyltransferase 1a antibodyAnti-Ntm1a Cleaved Into - N-Terminal Xaa-Pro-Lys N-Methyltransferase 1 - N-Terminally Processed antibodyAnti-NTMT1 antibodyAnti-C9orf32 antibodyAnti-METTL11A antibodyAnti-NRMT antibodyAnti-NRMT1 antibodyAnti-AD-003 antibody |
Information sourced from Uniprot.org