| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NTMT1 (1-223) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NTMT1 |
| Gene ID: |
28989 |
| Uniprot ID: |
NTM1A_HUMAN |
| Immunogen Region: |
1-223 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human NTMT1 (NP_054783.2). |
| Immunogen Sequence: |
MTSEVIEDEKQFYSKAKTYW KQIPPTVDGMLGGYGHISSI DINSSRKFLQRFLREGPNKT GTSCALDCGAGIGRITKRLL LPLFREVDMVDITEDFLVQA KTYLGEEGKRVRNYFCCGLQ DFTPEPDSYDVIWIQWVIGH LTDQHLAEFLRRCKGSLRPN GIIVIKDNMAQEGVILDDVD SSVCRDLDVVRRIICSAGLS LLAEERQENLPDEIYHVYSF ALR |
| Function | Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif Ala/Gly/Pro/Ser-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the Ala/Gly/Ser-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by NTMT2-mediated monomethylation. Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1. |
| Protein Name | N-Terminal Xaa-Pro-Lys N-Methyltransferase 1Alpha N-Terminal Protein Methyltransferase 1aMethyltransferase-Like Protein 11aN-Terminal Rcc1 MethyltransferaseX-Pro-Lys N-Terminal Protein Methyltransferase 1aNtm1a Cleaved Into - N-Terminal Xaa-Pro-Lys N-Methyltransferase 1 - N-Terminally Processed |
| Cellular Localisation | NucleusPredominantly Nuclear |
| Alternative Antibody Names | Anti-N-Terminal Xaa-Pro-Lys N-Methyltransferase 1 antibodyAnti-Alpha N-Terminal Protein Methyltransferase 1a antibodyAnti-Methyltransferase-Like Protein 11a antibodyAnti-N-Terminal Rcc1 Methyltransferase antibodyAnti-X-Pro-Lys N-Terminal Protein Methyltransferase 1a antibodyAnti-Ntm1a Cleaved Into - N-Terminal Xaa-Pro-Lys N-Methyltransferase 1 - N-Terminally Processed antibodyAnti-NTMT1 antibodyAnti-C9orf32 antibodyAnti-METTL11A antibodyAnti-NRMT antibodyAnti-NRMT1 antibodyAnti-AD-003 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89668/358164/STJ11100730_1__42931.1713122467.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AK4 knockout (KO) HeLa cells, using [KO Validated] AK4 Rabbit polyclonal antibody (STJ11100730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
