Host: |
Rabbit |
Applications: |
WB/ICC/IF |
Reactivity: |
Human |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Rabbit monoclonal antibody anti-NTH1 (24-126 aa) is suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence research applications. |
Clonality: |
Monoclonal |
Clone ID: |
R08-8E9 |
Conjugation: |
Unconjugated |
Isotype: |
IgG |
Formulation: |
50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
Purification: |
Affinity Purified |
Concentration: |
0.3 mg/ml |
Dilution Range: |
WB 1:500-1:1000IF 1:50-1:200 |
Storage Instruction: |
Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Gene Symbol: |
NTHL1 |
Gene ID: |
4913 |
Uniprot ID: |
NTH_HUMAN |
Immunogen Region: |
24-126 aa |
Immunogen: |
Recombinant protein of human NTH1 |
Tissue Specificity | Widely expressed with highest levels in heart and lowest levels in lung and liver. |
Post Translational Modifications | Ubiquitinated by TRIM26.leading to proteasomal degradation. |
Function | Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Has also 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption. |
Protein Name | Endonuclease Iii-Like Protein 1Hnth1Bifunctional Dna N-Glycosylase/Dna-(Apurinic Or Apyrimidinic Site LyaseDna Glycosylase/Ap Lyase |
Database Links | Reactome: R-HSA-110328Reactome: R-HSA-110329Reactome: R-HSA-110357Reactome: R-HSA-9630221Reactome: R-HSA-9630222 |
Cellular Localisation | NucleusMitochondrion |
Alternative Antibody Names | Anti-Endonuclease Iii-Like Protein 1 antibodyAnti-Hnth1 antibodyAnti-Bifunctional Dna N-Glycosylase/Dna-(Apurinic Or Apyrimidinic Site Lyase antibodyAnti-Dna Glycosylase/Ap Lyase antibodyAnti-NTHL1 antibodyAnti-NTH1 antibodyAnti-OCTS3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance