| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NSD2 |
| Gene ID: | 7468 |
| Uniprot ID: | NSD2_HUMAN |
| Immunogen Region: | 1-240 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human WHSC1 (NP_579877.1). |
| Immunogen Sequence: | MEFSIKQSPLSVQSVVKCIK MKQAPEILGSANGKTPSCEV NRECSVFLSKAQLSSSLQEG VMQKFNGHDALPFIPADKLK DLTSRVFNGEPGAHDAKLRF ESQEMKGIGTPPNTTPIKNG SPEIKLKITKTYMNGKPLFE SSICGDSAADVSQSEENGQK PENKARRNRKRSIKYDSLLE QGLVEAALVSKISSPSDKKI PAKKESCPNTGRDKDHLLKY NVGDLVWSKVSGYPWWPCM |
| Tissue Specificity | Widely expressed. Predominantly expressed in thymus and testis. |
| Function | Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2). Also monomethylates nucleosomal histone H3 at 'Lys-36' (H3K36me) in vitro. Does not trimethylate nucleosomal histone H3 at 'Lys-36' (H3K36me3). However, specifically trimethylates histone H3 at 'Lys-36' (H3K36me3) at euchromatic regions in embryonic stem (ES) cells. By methylating histone H3 at 'Lys-36', involved in the regulation of gene transcription during various biological processes. In ES cells, associates with developmental transcription factors such as SALL1 and represses inappropriate gene transcription mediated by histone deacetylation. During heart development, associates with transcription factor NKX2-5 to repress transcription of NKX2-5 target genes. Plays an essential role in adipogenesis, by regulating expression of genes involved in pre-adipocyte differentiation. During T-cell receptor (TCR) and CD28-mediated T-cell activation, promotes the transcription of transcription factor BCL6 which is required for follicular helper T (Tfh) cell differentiation. During B-cell development, required for the generation of the B1 lineage. During B2 cell activation, may contribute to the control of isotype class switch recombination (CRS), splenic germinal center formation, and the humoral immune response. Plays a role in class switch recombination of the immunoglobulin heavy chain (IgH) locus during B-cell activation. By regulating the methylation of histone H3 at 'Lys-36' and histone H4 at 'Lys-20' at the IgH locus, involved in TP53BP1 recruitment to the IgH switch region and promotes the transcription of IgA. Isoform 1: Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2). Isoform 4: Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2). Methylation of histone H3 at 'Lys-27' is controversial. Mono-, di- or tri-methylates histone H3 at 'Lys-27' (H3K27me, H3K27me2 and H3K27me3). Does not methylate histone H3 at 'Lys-27'. May act as a transcription regulator that binds DNA and suppresses IL5 transcription through HDAC recruitment. |
| Protein Name | Histone-Lysine N-Methyltransferase Nsd2 Multiple Myeloma Set Domain-Containing Protein Mmset Nuclear Set Domain-Containing Protein 2 Protein Trithorax-5 Wolf-Hirschhorn Syndrome Candidate 1 Protein |
| Database Links | Reactome: R-HSA-3214841 Reactome: R-HSA-5693565 Reactome: R-HSA-5693571 Reactome: R-HSA-5693607 Reactome: R-HSA-69473 |
| Cellular Localisation | Nucleus Chromosome In Embryonic Stem (Es) Cells Localizes To Small Foci Probably Corresponding To Euchromatin In B-Cells Localizes To Ig Heavy Chain Switch Region During Class Switch Recombination Isoform 1: Nucleus Isoform 3: Nucleus Isoform 4: Cytoplasm Nucleolus |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Nsd2 antibody Anti-Multiple Myeloma Set Domain-Containing Protein antibody Anti-Mmset antibody Anti-Nuclear Set Domain-Containing Protein 2 antibody Anti-Protein Trithorax-5 antibody Anti-Wolf-Hirschhorn Syndrome Candidate 1 Protein antibody Anti-NSD2 antibody Anti-KIAA1090 antibody Anti-MMSET antibody Anti-TRX5 antibody Anti-WHSC1 antibody |
Information sourced from Uniprot.org