• Western blot analysis of various lysates, using NR5A2 antibody (STJ28333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using NR5A2 rabbit polyclonal antibody (STJ28333) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of mouse liver cells using NR5A2 rabbit polyclonal antibody (STJ28333) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NR5A2 antibody (300-541) (STJ28333)

SKU:
STJ28333

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NR5A2 (300-541) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NR5A2
Gene ID: 2494
Uniprot ID: NR5A2_HUMAN
Immunogen Region: 300-541
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-541 of human NR5A2 (NP_995582.1).
Immunogen Sequence: SIPHLILELLKCEPDEPQVQ AKIMAYLQQEQANRSKHEKL STFGLMCKMADQTLFSIVEW ARSSIFFRELKVDDQMKLLQ NCWSELLILDHIYRQVVHGK EGSIFLVTGQQVDYSIIASQ AGATLNNLMSHAQELVAKLR SLQFDQREFVCLKFLVLFSL DVKNLENFQLVEGVQEQVNA ALLDYTMCNYPQQTEKFGQL LLRLPEIRAISMQAEEYLYY KHLNGDVPYNNLLIEMLHA
Tissue Specificity Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells.
Post Translational Modifications Sumoylated by SUMO1 at Lys-270 during the hepatic acute phase response, leading to promote interaction with GPS2 and prevent N-Cor corepressor complex dissociation.
Function Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38.
Protein Name Nuclear Receptor Subfamily 5 Group A Member 2
Alpha-1-Fetoprotein Transcription Factor
B1-Binding Factor
Hb1f
Cyp7a Promoter-Binding Factor
Hepatocytic Transcription Factor
Liver Receptor Homolog 1
Lrh-1
Database Links Reactome: R-HSA-210747 O00482-1
Reactome: R-HSA-383280
Reactome: R-HSA-4090294 O00482-2
Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Receptor Subfamily 5 Group A Member 2 antibody
Anti-Alpha-1-Fetoprotein Transcription Factor antibody
Anti-B1-Binding Factor antibody
Anti-Hb1f antibody
Anti-Cyp7a Promoter-Binding Factor antibody
Anti-Hepatocytic Transcription Factor antibody
Anti-Liver Receptor Homolog 1 antibody
Anti-Lrh-1 antibody
Anti-NR5A2 antibody
Anti-B1F antibody
Anti-CPF antibody
Anti-FTF antibody

Information sourced from Uniprot.org

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