• Western blot analysis of extracts of various cell lines, using PXR antibody (STJ119280) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of C6 cells using PXR Polyclonal Antibody (STJ119280) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 600 Mu g extracts of mouse stomach cells using 3 Mu g PXR antibody (STJ119280). Western blot was performed from the immunoprecipitate using PXR antibody (STJ119280) at a dilution of 1:500.

Anti-NR1I2 antibody (135-230) (STJ119280)

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STJ119280

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PXR (135-230) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NR1I2
Gene ID: 8856
Uniprot ID: NR1I2_HUMAN
Immunogen Region: 135-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 135-230 of human PXR (NP_003880.3).
Immunogen Sequence: TQPLGVQGLTEEQRMMIREL MDAQMKTFDTTFSHFKNFRL PGVLSSGCELPESLQAPSRE EAAKWSQVRKDLCSLKVSLQ LRGEDGSVWNYKPPAD
Tissue Specificity Expressed in liver, colon and small intestine.
Function Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.
Protein Name Nuclear Receptor Subfamily 1 Group I Member 2
Orphan Nuclear Receptor Par1
Orphan Nuclear Receptor Pxr
Pregnane X Receptor
Steroid And Xenobiotic Receptor
Sxr
Database Links Reactome: R-HSA-383280
Reactome: R-HSA-4090294 O75469-1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Receptor Subfamily 1 Group I Member 2 antibody
Anti-Orphan Nuclear Receptor Par1 antibody
Anti-Orphan Nuclear Receptor Pxr antibody
Anti-Pregnane X Receptor antibody
Anti-Steroid And Xenobiotic Receptor antibody
Anti-Sxr antibody
Anti-NR1I2 antibody
Anti-PXR antibody

Information sourced from Uniprot.org

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