• Immunohistochemistry analysis of NQO2 in paraffin-embedded Mouse kidney using NQO2 Rabbit polyclonal antibody (STJ113339) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NQO2 in paraffin-embedded Human liver using NQO2 Rabbit polyclonal antibody (STJ113339) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using NQO2 Rabbit polyclonal antibody (STJ113339) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NQO2 antibody (1-231) (STJ113339)

SKU:
STJ113339

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NQO2 (1-231) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NQO2
Gene ID: 4835
Uniprot ID: NQO2_HUMAN
Immunogen Region: 1-231
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-231 of human NQO2 (NP_000895.2).
Immunogen Sequence: MAGKKVLIVYAHQEPKSFNG SLKNVAVDELSRQGCTVTVS DLYAMNLEPRATDKDITGTL SNPEVFNYGVETHEAYKQRS LASDITDEQKKVREADLVIF QFPLYWFSVPAILKGWMDRV LCQGFAFDIPGFYDSGLLQG KLALLSVTTGGTAEMYTKTG VNGDSRYFLWPLQHGTLHFC GFKVLAPQISFAPEIASEEE RKGMVAAWSQRLQTIWKEEP IPCTAHWHFGQ
Function The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Protein Name Ribosyldihydronicotinamide Dehydrogenase Quinone
Nrh Dehydrogenase Quinone 2
Nrh -Quinone Oxidoreductase 2
Quinone Reductase 2
Qr2
Database Links Reactome: R-HSA-211945
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ribosyldihydronicotinamide Dehydrogenase Quinone antibody
Anti-Nrh Dehydrogenase Quinone 2 antibody
Anti-Nrh -Quinone Oxidoreductase 2 antibody
Anti-Quinone Reductase 2 antibody
Anti-Qr2 antibody
Anti-NQO2 antibody
Anti-NMOR2 antibody

Information sourced from Uniprot.org

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