| Short Description : | Rabbit polyclonal NPHS1 (1019-1241) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NPHS1 |
| Gene ID: | 4868 |
| Uniprot ID: | NPHN_HUMAN |
| Immunogen Region: | 1019-1241 |
| Immunogen Sequence: | LGDSGLADKGTQLPITTPGL HQPSGEPEDQLPTEPPSGPS GLPLLPVLFALGGLLLLSNA SCVGGVLWQRRLRRLAEGIS EKTEAGSEEDRVRNEYEESQ WTGERDTQSSTVSTTEAEPY YRSLRDFSPQLPPTQEEVSY SRGFTGEDEDMAFPGHLYDE VERTYPPSGAWGPLYDEVQM GPWDLHWPEDTYQDPRGIYD QVAGDLDTLEPDSLPFELRG HLV |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1019-1241 of human NPHS1 (NP_004637.1). |
| Tissue Specificity | Specifically expressed in podocytes of kidney glomeruli. |
| Post Translational Modifications | Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. Tyrosine phosphorylation is reduced by high glucose levels. Dephosphorylated by tensin TNS2 which leads to reduced binding of NPHN1 to PIK3R1. |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. |
| Protein Name | NephrinRenal Glomerulus-Specific Cell Adhesion Receptor |
| Database Links | Reactome: R-HSA-373753 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinPredominantly Located At Podocyte Slit Diaphragm Between Podocyte Foot ProcessesAlso Associated With Podocyte Apical Plasma Membrane |
| Alternative Antibody Names | Anti-Nephrin antibodyAnti-Renal Glomerulus-Specific Cell Adhesion Receptor antibodyAnti-NPHS1 antibodyAnti-NPHN antibody |
Information sourced from Uniprot.org
