| Host: | Goat |
| Applications: | Pep-ELISA/IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-NOXO1 (C-Term) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000. WB: Preliminary experiments gave no signal but low background in Hela and Human Heart lysates at up to 1µg/ml. IHC: In paraffin embedded Human Kidney Cortex shows cytoplasmic stain in |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | NOXO1 |
| Gene ID: | 124056 |
| Uniprot ID: | NOXO1_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_653204.1; NP_751907.1; NP_751907.1 |
| Specificity: | This antibody is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1). |
| Immunogen Sequence: | RGCVDSVPHPTTEQ |
| Function | Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity. |
| Protein Name | Nadph Oxidase Organizer 1 Nadph Oxidase Regulatory Protein Nox Organizer 1 Nox-Organizing Protein 1 Sh3 And Px Domain-Containing Protein 5 |
| Database Links | Reactome: R-HSA-5668599 Reactome: R-HSA-9013149 Reactome: R-HSA-9013423 Reactome: R-HSA-9673324 |
| Cellular Localisation | Isoform 3: Cell Membrane Peripheral Membrane Protein Cytoplasmic Side Isoform 3 Associates With The Plasma Membrane In A Lipid-Dependent Manner |
| Alternative Antibody Names | Anti-Nadph Oxidase Organizer 1 antibody Anti-Nadph Oxidase Regulatory Protein antibody Anti-Nox Organizer 1 antibody Anti-Nox-Organizing Protein 1 antibody Anti-Sh3 And Px Domain-Containing Protein 5 antibody Anti-NOXO1 antibody Anti-P41NOX antibody Anti-SH3PXD5 antibody |
Information sourced from Uniprot.org