| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NOX5 |
| Gene ID: |
134826889/79400 |
| Uniprot ID: |
NOX5_HUMAN |
| Immunogen Region: |
661-765 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 661-765 of human NOX5 (NP_078781.3). |
| Immunogen Sequence: |
AEEAQYGRFLELHMYMTSAL GKNDMKAIGLQMALDLLANK EKKDSITGLQTRTQPGRPDW SKVFQKVAAEKKGKVQVFFC GSPALAKVLKGHCEKFGFRF FQENF |
| Tissue Specificity | Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1: Expressed in spleen. Expressed in endothelial cells, pulmonary artery smooth muscle cells and epithelial colorectal adenocarcinoma cells. Isoform v2: Expressed in microvascular endothelial cells (at protein level). Expressed in testis. Expressed in endothelial cells and pulmonary artery smooth muscle cells. Isoform v3: Expressed in pulmonary artery smooth muscle cells and epithelial colorectal adenocarcinoma cells. Isoform v4: Expressed in endothelial cells and pulmonary artery smooth muscle cells. Isoform v5: Expressed in microvascular endothelial cells (at protein level). |
| Post Translational Modifications | Isoform v2: Phosphorylation at Ser-475 by CaMK2 and at Ser-490, Thr-494 and Ser-498 by PKC/PRKCA positively regulates its catalytic activity. Isoform v2: S-nitrosylation in response to nitric oxide inhibits its catalytic activity. |
| Function | Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. May play a role in cell growth and apoptosis. Isoform v2: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contributes to endothelial response to thrombin. Regulates redox-dependent processes in lymphocytes and spermatozoa. Isoform v1: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Isoform v5: This isoform lacks calcium-binding domains and was showed to present a NADPH oxidase activity in a calcium-independent manner. May be involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. However another study showed an absence of oxidase activity. Subject to rapid degradation. Isoform v3: Lacks calcium-dependent NADPH oxidase activity. Isoform v4: Lacks calcium-dependent NADPH oxidase activity. |
| Protein Name | Nadph Oxidase 5 |
| Database Links | Reactome: R-HSA-3299685 |
| Cellular Localisation | Isoform V2: Endoplasmic ReticulumCell MembraneMulti-Pass Membrane ProteinCalcium-Sensitive Association And Dissociation Between The N- And C-Terminal Domains Appears To Facilitate Its Localization To The Cell MembraneIsoform V5: Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Nadph Oxidase 5 antibodyAnti-NOX5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
