| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NOSIP |
| Gene ID: | 51070 |
| Uniprot ID: | NOSIP_HUMAN |
| Immunogen Region: | 1-301 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human NOSIP (NP_057037.1). |
| Immunogen Sequence: | MTRHGKNCTAGAVYTYHEKK KDTAASGYGTQNIRLSRDAV KDFDCCCLSLQPCHDPVVTP DGYLYEREAILEYILHQKKE IARQMKAYEKQRGTRREEQK ELQRAASQDHVRGFLEKESA IVSRPLNPFTAKALSGTSPD DVQPGPSVGPPSKDKDKVLP SFWIPSLTPEAKATKLEKPS RTVTCPMSGKPLRMSDLTPV HFTPLDSSVDRVGLITRSER YVCAVTRDSLSNATPCAVL |
| Tissue Specificity | Expressed in heart, brain and lung. Present in endothelial cells (at protein level). |
| Function | E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB. Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity. |
| Protein Name | Nitric Oxide Synthase-Interacting ProteinE3 Ubiquitin-Protein Ligase NosipRing-Type E3 Ubiquitin Transferase NosipEnos-Interacting Protein |
| Database Links | Reactome: R-HSA-203754 |
| Cellular Localisation | CytoplasmNucleusTranslocates From Nucleus To Cytoplasm In The G2 Phase Of The Cell Cycle |
| Alternative Antibody Names | Anti-Nitric Oxide Synthase-Interacting Protein antibodyAnti-E3 Ubiquitin-Protein Ligase Nosip antibodyAnti-Ring-Type E3 Ubiquitin Transferase Nosip antibodyAnti-Enos-Interacting Protein antibodyAnti-NOSIP antibodyAnti-CGI-25 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and NOSIP knockout (KO) 293T cells using [KO Validated] NOSIP Rabbit polyclonal antibody (STJ11104394) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/177038/368258/STJ11104394_1__88977.1713133758.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NOSIP knockout (KO) 293T cells using [KO Validated] NOSIP Rabbit polyclonal antibody (STJ11104394) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
