Host: | Rabbit |
Applications: | WB/ELISA |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | NOSIP |
Gene ID: | 51070 |
Uniprot ID: | NOSIP_HUMAN |
Immunogen Region: | 1-301 |
Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human NOSIP (NP_057037.1). |
Immunogen Sequence: | MTRHGKNCTAGAVYTYHEKK KDTAASGYGTQNIRLSRDAV KDFDCCCLSLQPCHDPVVTP DGYLYEREAILEYILHQKKE IARQMKAYEKQRGTRREEQK ELQRAASQDHVRGFLEKESA IVSRPLNPFTAKALSGTSPD DVQPGPSVGPPSKDKDKVLP SFWIPSLTPEAKATKLEKPS RTVTCPMSGKPLRMSDLTPV HFTPLDSSVDRVGLITRSER YVCAVTRDSLSNATPCAVL |
Tissue Specificity | Expressed in heart, brain and lung. Present in endothelial cells (at protein level). |
Function | E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB. Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity. |
Protein Name | Nitric Oxide Synthase-Interacting ProteinE3 Ubiquitin-Protein Ligase NosipRing-Type E3 Ubiquitin Transferase NosipEnos-Interacting Protein |
Database Links | Reactome: R-HSA-203754 |
Cellular Localisation | CytoplasmNucleusTranslocates From Nucleus To Cytoplasm In The G2 Phase Of The Cell Cycle |
Alternative Antibody Names | Anti-Nitric Oxide Synthase-Interacting Protein antibodyAnti-E3 Ubiquitin-Protein Ligase Nosip antibodyAnti-Ring-Type E3 Ubiquitin Transferase Nosip antibodyAnti-Enos-Interacting Protein antibodyAnti-NOSIP antibodyAnti-CGI-25 antibody |
Information sourced from Uniprot.org