| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NOD1 (1-270) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NOD1 |
| Gene ID: |
10392 |
| Uniprot ID: |
NOD1_HUMAN |
| Immunogen Region: |
1-270 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NOD1 (NP_006083.1). |
| Immunogen Sequence: |
MEEQGHSEMEIIPSESHPHI QLLKSNRELLVTHIRNTQCL VDNLLKNDYFSAEDAEIVCA CPTQPDKVRKILDLVQSKGE EVSEFFLYLLQQLADAYVDL RPWLLEIGFSPSLLTQSKVV VNTDPVSRYTQQLRHHLGRD SKFVLCYAQKEELLLEEIYM DTIMELVGFSNESLGSLNSL ACLLDHTTGILNEQGETIFI LGDAGVGKSMLLQRLQSLWA TGRLDAGVKFFFHFRCRMF |
| Tissue Specificity | Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon. |
| Post Translational Modifications | Palmitoylated. Palmitoylation is required for proper recruitment to the bacterial entry site and hence for proper signaling upon cognate peptidoglycan detection. Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF34 promotes proteasomal degradation and thereby negatively regulates NOD1 for instance in NF-kappa-B activation. Degraded via selective autophagy following interaction with IRGM. IRGM promotes NOD1-RIPK2 RIPosome recruitment to autophagosome membranes, promoting their SQSTM1/p62-dependent autophagic degradation. |
| Function | Pattern recognition receptor (PRR) that detects bacterial peptidoglycan fragments and other danger signals and thus participates in both innate and adaptive immune responses. Specifically recognizes and binds gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP), a dipeptide present in peptidoglycan of Gram-negative bacteria. Preferentially binds iE-DAP in tripeptide-containing muropeptides (MurNAc-TriDAP or TriDAP). Ligand binding triggers oligomerization that facilitates the binding and subsequent activation of the proximal adapter receptor-interacting RIPK2. Following recruitment, RIPK2 undergoes 'Met-1'- (linear) and 'Lys-63'-linked polyubiquitination by E3 ubiquitin-protein ligases XIAP, BIRC2, BIRC3 and the LUBAC complex, becoming a scaffolding protein for downstream effectors, triggering activation of the NF-kappa-B and MAP kinases signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response. Also acts as a regulator of antiviral response elicited by dsRNA and the expression of RLR pathway members by targeting IFIH1 and TRAF3 to modulate the formation of IFIH1-MAVS and TRAF3-MAVS complexes leading to increased transcription of type I IFNs. Also acts as a regulator of autophagy via its interaction with ATG16L1, possibly by recruiting ATG16L1 at the site of bacterial entry. Besides recognizing pathogens, also involved in the endoplasmic reticulum stress response: acts by sensing and binding to the cytosolic metabolite sphingosine-1-phosphate generated in response to endoplasmic reticulum stress, initiating an inflammation process that leads to activation of the NF-kappa-B and MAP kinases signaling. In addition, plays a role in insulin trafficking in beta cells in a cell-autonomous manner. Mechanistically, upon recognizing cognate ligands, NOD1 and RIPK2 localize to insulin vesicles where they recruit RAB1A to direct insulin trafficking through the cytoplasm. Isoform 3: In contrast to isoform 1, does not efficiently recognize and bind gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP) ligand. |
| Protein Name | Nucleotide-Binding Oligomerization Domain-Containing Protein 1Hnod1Caspase Recruitment Domain-Containing Protein 4 |
| Database Links | Reactome: R-HSA-168638Reactome: R-HSA-445989Reactome: R-HSA-450302Reactome: R-HSA-450321Reactome: R-HSA-5689896Reactome: R-HSA-9020702Reactome: R-HSA-9705671 |
| Cellular Localisation | Cell MembraneLipid-AnchorApical Cell MembraneBasolateral Cell MembraneCytoplasmDetected In The Cytoplasm And At The Cell MembraneFollowing Bacterial InfectionLocalizes To Bacterial Entry Sites In The Cell MembraneRecruited To The Basolateral And Apical Membranes In Polarized Epithelial Cells |
| Alternative Antibody Names | Anti-Nucleotide-Binding Oligomerization Domain-Containing Protein 1 antibodyAnti-Hnod1 antibodyAnti-Caspase Recruitment Domain-Containing Protein 4 antibodyAnti-NOD1 antibodyAnti-CARD4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
