• Immunofluorescence analysis of NIH/3T3 cells using NMNAT3 Rabbit polyclonal antibody (STJ116675) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using NMNAT3 Rabbit polyclonal antibody (STJ116675) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NMNAT3 Rabbit polyclonal antibody (STJ116675) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NMNAT3 antibody (1-215) (STJ116675)

SKU:
STJ116675

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NMNAT3 (1-215) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NMNAT3
Gene ID: 349565
Uniprot ID: NMNA3_HUMAN
Immunogen Region: 1-215
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human NMNAT3 (NP_835471.1).
Immunogen Sequence: MYQVIQGIISPVNDTYGKKD LAASHHRVAMARLALQTSDW IRVDPWESEQAQWMETVKVL RHHHSKLLRSPPQMEGPDHG KALFSTPAAVPELKLLCGAD VLKTFQTPNLWKDAHIQEIV EKFGLVCVGRVGHDPKGYIA ESPILRMHQHNIHLAKEPVQ NEISATYIRRALGQGQSVKY LIPDAVITYIKDHGLYTKGS TWKGKSTQSTEGKTS
Tissue Specificity Expressed in lung and spleen with lower levels in placenta and kidney.
Function Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form.nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD(+), NADH, NaAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following injury.
Protein Name Nicotinamide/Nicotinic Acid Mononucleotide Adenylyltransferase 3
Nmn/Namn Adenylyltransferase 3
Nicotinamide-Nucleotide Adenylyltransferase 3
Nmn Adenylyltransferase 3
Nicotinate-Nucleotide Adenylyltransferase 3
Namn Adenylyltransferase 3
Pyridine Nucleotide Adenylyltransferase 3
Pnat-3
Database Links Reactome: R-HSA-196807
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Nicotinamide/Nicotinic Acid Mononucleotide Adenylyltransferase 3 antibody
Anti-Nmn/Namn Adenylyltransferase 3 antibody
Anti-Nicotinamide-Nucleotide Adenylyltransferase 3 antibody
Anti-Nmn Adenylyltransferase 3 antibody
Anti-Nicotinate-Nucleotide Adenylyltransferase 3 antibody
Anti-Namn Adenylyltransferase 3 antibody
Anti-Pyridine Nucleotide Adenylyltransferase 3 antibody
Anti-Pnat-3 antibody
Anti-NMNAT3 antibody
Anti-FKSG76 antibody

Information sourced from Uniprot.org

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