| Short Description : | Rabbit polyclonal NKX2-5 (1-135) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NKX2-5 |
| Gene ID: | 1482 |
| Uniprot ID: | NKX25_HUMAN |
| Immunogen Region: | 1-135 |
| Immunogen Sequence: | MFPSPALTPTPFSVKDILNL EQQQRSLAAAGELSARLEAT LAPSSCMLAAFKPEAYAGPE AAAPGLPELRAELGRAPSPA KCASAFPAAPAFYPRAYSDP DPAKDPRAEKKELCALQKAV ELEKTEADNAERPRA |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human NKX2-5 (NP_004378.1). |
| Tissue Specificity | Expressed only in the heart. |
| Function | Transcription factor required for the development of the heart and the spleen. During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4. May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal. Binds to the core DNA motif of NPPA promoter. Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression. Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia. |
| Protein Name | Homeobox Protein Nkx-2.5Cardiac-Specific HomeoboxHomeobox Protein CsxHomeobox Protein Nk-2 Homolog E |
| Database Links | Reactome: R-HSA-2032785Reactome: R-HSA-5578768Reactome: R-HSA-9733709 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Homeobox Protein Nkx-2.5 antibodyAnti-Cardiac-Specific Homeobox antibodyAnti-Homeobox Protein Csx antibodyAnti-Homeobox Protein Nk-2 Homolog E antibodyAnti-NKX2-5 antibodyAnti-CSX antibodyAnti-NKX2.5 antibodyAnti-NKX2E antibody |
Information sourced from Uniprot.org
