Western blot analysis of various lysates using NKD1 Rabbit polyclonal antibody (STJ119828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NKD1 antibody (1-100) (STJ119828)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ119828
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General Information

Short DescriptionRabbit polyclonal NKD1 (1-100) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolNKD1
Gene ID85407
Uniprot IDNKD1_HUMAN
Immunogen Region1-100
Immunogen SequenceMGKLHSKPAAVCKRRESPEG DSFAVSAAWARKGIEEWIGR QRCPGGVSGPRQLRLAGTIG RSTRELVGDVLRDTLSEEEE DDFRLEVALPPEKTDGLGSG
SpecificityA synthetic peptide corresponding to a sequence within amino acids 1-100 of human NKD1 (NP_149110.1).

Additional Info

Tissue Specificity Expressed in colon, heart, kidney, leukocyte, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine and spleen.
Function Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity.
Protein Name Protein Naked Cuticle Homolog 1
Naked-1
Hnkd
Hnkd1
Database Links
Cellular Localisation Cell Membrane
Cytoplasm
Alternative Antibody Names Anti-Protein Naked Cuticle Homolog 1 antibody
Anti-Naked-1 antibody
Anti-Hnkd antibody
Anti-Hnkd1 antibody
Anti-NKD1 antibody
Anti-NKD antibody
Anti-PP7246 antibody

Information sourced from Uniprot.org

Citations

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