Anti-NIN antibody (STJA0009761)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human Ninein
STJA0009761
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General Information

Short DescriptionRabbit polyclonal anti-Ninein for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-2000
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolNIN
Gene ID51199
Uniprot IDNIN_HUMAN
ImmunogenSynthesized peptide derived from human Ninein
SpecificityThis antibody detects endogenous levels of Ninein at Human, Mouse

Additional Info

Post Translational Modifications Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/ Aurora kinase B.
Function Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. Required for centriole organization and microtubule anchoring at the mother centriole.
Protein Name Ninein
Hninein
Glycogen Synthase Kinase 3 Beta-Interacting Protein
Gsk3b-Interacting Protein
Database Links
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Component Of The Core Centrosome
Arranged In A Tubular Conformation With An Open And A Closed End Within The Centrosome
In The Mother Centrosome
It Localizes At Both Ends Of The Centrosome Tube
Including The Site Of Centrosome Duplication
While In The Daughter Centrosome It Is Present Only At The Closed End
Requires Pcm1 For Centrosome Localization
Localizes To The Subdistal Appendage Region Of The Centriole In A Dctn1-Dependent Manner
Isoform 6: Cytoplasm
Seems To Have A Dominant-Negative Effect On Localization Of Other Isoforms
Promoting Their Dissociation From The Centrosome
Alternative Antibody Names Anti-Ninein antibody
Anti-Hninein antibody
Anti-Glycogen Synthase Kinase 3 Beta-Interacting Protein antibody
Anti-Gsk3b-Interacting Protein antibody
Anti-NIN antibody
Anti-KIAA1565 antibody

Information sourced from Uniprot.org

Citations

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