• Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NIFK Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night
  • Immunofluorescence analysis of HUVEC cells, using NIFK Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-NIFK antibody (200-249) (STJ94492)

SKU:
STJ94492

Current Stock:
Host: Rabbit
Applications: IHC/IF/WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MKI67 FHA domain-interacting nucleolar phosphoprotein (200-249) is suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NIFK
Gene ID: 84365
Uniprot ID: MK67I_HUMAN
Immunogen Region: 200-249
Specificity: NIFK Polyclonal Antibody detects endogenous levels of NIFK protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human NIFK. AA range:200-249
Post Translational Modifications Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
Protein Name Mki67 Fha Domain-Interacting Nucleolar Phosphoprotein
Nucleolar Phosphoprotein Nopp34
Nucleolar Protein Interacting With The Fha Domain Of Pki-67
Hnifk
Cellular Localisation Nucleus
Nucleolus
Chromosome
Localizes To Mitotic Chromosomes In Conjunction With Mki67
Alternative Antibody Names Anti-Mki67 Fha Domain-Interacting Nucleolar Phosphoprotein antibody
Anti-Nucleolar Phosphoprotein Nopp34 antibody
Anti-Nucleolar Protein Interacting With The Fha Domain Of Pki-67 antibody
Anti-Hnifk antibody
Anti-NIFK antibody
Anti-MKI67IP antibody
Anti-NOPP34 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance