| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-NHERF2-Isoform a (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 38kDa band observed in human kidney lysates (calculated MW of 37.4kDa according to BAA33216.1). Recommended concentration: 0.3-1µg/ml. IHC: In paraffin embedded Human Kidney |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | NHERF2 |
| Gene ID: | 9351 |
| Uniprot ID: | NHRF2_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_001123484.1 |
| Specificity: | Please note that this variant has an extra insertion of 11 residues compared to NP_004776.3. The immunizing peptide represents this insertion. |
| Immunogen Sequence: | DGSAWKQDPFQ |
| Function | Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus. |
| Protein Name | Na(+/H(+ Exchange Regulatory Cofactor Nhe-Rf2 Nherf-2 Nhe3 Kinase A Regulatory Protein E3karp Sry-Interacting Protein 1 Sip-1 Sodium-Hydrogen Exchanger Regulatory Factor 2 Solute Carrier Family 9 Isoform A3 Regulatory Factor 2 Tyrosine Kinase Activator Protein 1 Tka-1 |
| Cellular Localisation | Endomembrane System Peripheral Membrane Protein Nucleus Apical Cell Membrane Localizes With Ezr And Podxl At The Apical Cell Membrane Of Glomerular Epithelium Cells And The Sides Of The Food Processes Nuclear In A Punctate Pattern |
| Alternative Antibody Names | Anti-Na(+/H(+ Exchange Regulatory Cofactor Nhe-Rf2 antibody Anti-Nherf-2 antibody Anti-Nhe3 Kinase A Regulatory Protein E3karp antibody Anti-Sry-Interacting Protein 1 antibody Anti-Sip-1 antibody Anti-Sodium-Hydrogen Exchanger Regulatory Factor 2 antibody Anti-Solute Carrier Family 9 Isoform A3 Regulatory Factor 2 antibody Anti-Tyrosine Kinase Activator Protein 1 antibody Anti-Tka-1 antibody Anti-NHERF2 antibody Anti-SLC9A3R2 antibody |
Information sourced from Uniprot.org