STJ70795 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Anti-NHERF2-Isoform a antibody (Internal) (STJ70795)

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STJ70795-100
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-NHERF2-Isoform a (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 38kDa band observed in human kidney lysates (calculated MW of 37.4kDa according to BAA33216.1). Recommended concentration: 0.3-1µg/ml.
IHC: In paraffin embedded Human Kidney
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NHERF2
Gene ID: 9351
Uniprot ID: NHRF2_HUMAN
Immunogen Region: Internal
Accession Number: NP_001123484.1
Specificity: Please note that this variant has an extra insertion of 11 residues compared to NP_004776.3. The immunizing peptide represents this insertion.
Immunogen Sequence: DGSAWKQDPFQ
Function Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.
Protein Name Na(+/H(+ Exchange Regulatory Cofactor Nhe-Rf2
Nherf-2
Nhe3 Kinase A Regulatory Protein E3karp
Sry-Interacting Protein 1
Sip-1
Sodium-Hydrogen Exchanger Regulatory Factor 2
Solute Carrier Family 9 Isoform A3 Regulatory Factor 2
Tyrosine Kinase Activator Protein 1
Tka-1
Cellular Localisation Endomembrane System
Peripheral Membrane Protein
Nucleus
Apical Cell Membrane
Localizes With Ezr And Podxl At The Apical Cell Membrane Of Glomerular Epithelium Cells And The Sides Of The Food Processes
Nuclear
In A Punctate Pattern
Alternative Antibody Names Anti-Na(+/H(+ Exchange Regulatory Cofactor Nhe-Rf2 antibody
Anti-Nherf-2 antibody
Anti-Nhe3 Kinase A Regulatory Protein E3karp antibody
Anti-Sry-Interacting Protein 1 antibody
Anti-Sip-1 antibody
Anti-Sodium-Hydrogen Exchanger Regulatory Factor 2 antibody
Anti-Solute Carrier Family 9 Isoform A3 Regulatory Factor 2 antibody
Anti-Tyrosine Kinase Activator Protein 1 antibody
Anti-Tka-1 antibody
Anti-NHERF2 antibody
Anti-SLC9A3R2 antibody

Information sourced from Uniprot.org

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