Anti-NGEF antibody (C-Term) (STJA0005408)

SKU:
STJA0005408-100

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Host: Rabbit
Applications: WB/ICC
Reactivity: Chicken/Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ephexin-1 (C-Term) is suitable for use in Western Blot and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Antigen Affinity Purified
Dilution Range: WB 1:1000
IHC
ICC 1:50
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Ngef
Gene ID: 53972
Uniprot ID: NGEF_MOUSE
Immunogen Region: C-Term
Specificity: The antibody detects an 80 kDa* band corresponding to ephexin-1 in Western blots of human A431, Jurkat, and HUVEC cells, as well as rat PC12 cells and mouse brain tissue.
Immunogen: Ephexin-1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of mouse ephexin-1. This sequence has significant homology to the same region in human and rat ephexin-1, and has low homology to other known
Tissue Specificity Highly expressed in brain and to a lower extent in eye.
Post Translational Modifications Src-dependent phosphorylation at Tyr-177 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA. Phosphorylation by CDK5 upon EPHA4 activation by EFNA1 may regulate dendritic spine morphogenesis.
Function Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.
Protein Name Ephexin-1
Eph-Interacting Exchange Protein
Neuronal Guanine Nucleotide Exchange Factor
Database Links Reactome: R-MMU-193648
Reactome: -MMU-3928663
Reactome: -MMU-416482
Reactome: -MMU-8980692
Reactome: -MMU-9013148
Reactome: -MMU-9013149
Cellular Localisation Cytoplasm
Membrane
Cell Projection
Growth Cone
Associated With Membranes
Localizes To Axonal Growth Cones
Alternative Antibody Names Anti-Ephexin-1 antibody
Anti-Eph-Interacting Exchange Protein antibody
Anti-Neuronal Guanine Nucleotide Exchange Factor antibody
Anti-Ngef antibody

Information sourced from Uniprot.org

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